modified polyethylenimine: self assemble nanoparticle forming polymer for pdna delivery

نویسندگان

reza k. oskuee department of biotechnology, pharmaceutical and biotechnology research centers, school of pharmacy, mashhad university of medical sciences, mashhad, iran

ali dehshahri department of biotechnology, pharmaceutical and biotechnology research centers, school of pharmacy, mashhad university of medical sciences, mashhad, iran

wayne. t. shier department of medicinal chemistry, university of minnesota-twin cities, minneapolis, mn 55455, usa

mohammad ramezani department of biotechnology, pharmaceutical and biotechnology research centers, school of pharmacy, mashhad university of medical sciences, mashhad, iran

چکیده

objective polyethylenimine (pei), a readily available synthetic polycation which has high transfection efficiency owing to its buffering capacity was introduced for transfection a few years ago. but it has been reported that pei is cytotoxic in many cell lines. in this study, in order to enhance the transfection efficiency of 10 kda pei and reduce its toxicity, hydrophobic residues were grafted on pei. materials and methods pei polymers were modified by adding hydrophobic chains to the primary amines of pei in different degrees of grafting using bromoacetic acid derivatives with different lengths. these polymers were complexed with plasmid dna at different c/p ratios and the resulting nanoparticles were characterized by dynamic light scattering and etbr-dna binding assay to determine particle sizes and complex formation, respectively. cytotoxicity and transfection efficiency of the polymers were also tested in cultured neuro2a cell line. results dna condensation measurement revealed that the resulted polymers could form polyplexes with plasmid dna and they have the ability to condense dna in relatively low amounts of polymers. particle size measurement of polyplexes showed that they form particles in the size range of below 190 nm. transfection experiments showed that polymers which have been modified with hexanoic derivative could transfect pdna as good as 25 kda pei with the advantage of being much less toxic. conclusion results indicate that the structure modifications of pei accomplished in this study play a significant role in increasing the transfection efficiency and without inducing the cytotoxicity compared to pei itself.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Modified Polyethylenimine: Self Assemble Nanoparticle Forming Polymer for pDNA Delivery

Objective Polyethylenimine (PEI), a readily available synthetic polycation which has high transfection efficiency owing to its buffering capacity was introduced for transfection a few years ago. But it has been reported that PEI is cytotoxic in many cell lines. In this study, in order to enhance the transfection efficiency of 10 kDa PEI and reduce its toxicity, hydrophobic residues were grafte...

متن کامل

ShRNA-mediated knock-down of CD200 using the self-assembled nanoparticle-forming derivative of polyethylenimine

Objective(s): ShRNA-mediated silencing strategy is considered to be a potent therapeutic approach. The present study aimed to assess the ability of the previously prepared polyethylenimine (PEI) derivative for the shRNA knock-down of the CD200 gene on the cells obtained from the patients with chronic lymphocytic leukemia (CLL). Materials and Methods: Since there are several investigations...

متن کامل

Dynamic endosomolytic polymer conjugates for pDNA und siRNA delivery

Diese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet.

متن کامل

Ternary Complexes of Pdna, Polyethylenimine, and Γ-polyglutamic Acid for Gene Delivery Systems

We discovered a vector coated by γ-polyglutamic acid (γ-PGA) for effective and safe gene delivery. In order to develop a useful non-viral vector, we prepared several ternary complexes constructed with pDNA, polyethylenimine (PEI), and various polyanions, such as polyadenylic acid, polyinosinic-polycytidylic acid, α-polyaspartic acid, α-polyglutamic acid, and γ-PGA. The pDNA/PEI complex had a st...

متن کامل

Characterization and Evaluation of Novel Film Forming Polymer for Drug Delivery

In this study, Damar Batu (DB) − a novel biomaterial− is evaluated for its potential application in pharmaceutical coating. DB is a whitish to yellowish resin, characterized initially in terms of solubility, acid value, molecular weight (Mw), polydispersity index (Mw/Mn) and glass transition temperature (Tg). Neat plasticized films of DB are investigated for mechanical, water vapor transmission...

متن کامل

Characterization and Evaluation of Novel Film Forming Polymer for Drug Delivery

In this study, Damar Batu (DB) − a novel biomaterial− is evaluated for its potential application in pharmaceutical coating. DB is a whitish to yellowish resin, characterized initially in terms of solubility, acid value, molecular weight (Mw), polydispersity index (Mw/Mn) and glass transition temperature (Tg). Neat plasticized films of DB are investigated for mechanical, water vapor transmission...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۱، شماره ۱، صفحات ۳۳-۴۰

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023